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Fluorescence Filters

  • Exceptional optical density>OD6
  • Ideal for Point-of-Care Testing (POCT), Polymerase Chain Reaction (PCR), and fluorescence microscopes
  • Available in stock and custom versions
  • Standardized dimensions compatible with filter cubes from elite suppliers
  • Supporting miscellaneous fluorophores such as  FAM EX, Alexa Fluor, ATTO, BCECF, Cy5, Cy3, DAPI, DyLight, GFP, FITC, LysoTracker, mCherry, MitoTracker, Qdot, Quasar, Rhodamine, Texas Red, etc.
Inquire for custom product  
Code Central Wavelength Peak Transmission Rate Blocking Range Optical Density Application Bandwidth Unit Price Delivery Cart
416-001 470nm >90% 200-800nm OD6 FAM EX 30nm Inquire Inquire
416-002 525nm >90% 200-800nm OD6 FAM Em 20nm Inquire Inquire
416-003 523nm >90% 200-800nm OD6 Hex Ex 20nm Inquire Inquire
416-004 564nm >90% 200-800nm OD6 Hex Em 20nm Inquire Inquire
416-005 543nm >90% 200-800nm OD6 / 20nm Inquire Inquire
416-006 584nm >90% 200-800nm OD6 / 20nm Inquire Inquire
416-007 571nm >90% 200-800nm OD6 Rox Ex 20nm Inquire Inquire
416-008 612nm >90% 200-800nm OD6 Rox Em 20nm Inquire Inquire
416-009 624nm >90% 200-800nm OD6 Cy5 Ex 40nm Inquire Inquire
416-010 692nm >90% 200-800nm OD6 Cy5 Em 40nm Inquire Inquire
416-011 635nm >90% 200-800nm OD6 Cy5 Ex 40nm Inquire Inquire
416-012 683nm >90% 200-800nm OD6 Cy5 Fm 15nm Inquire Inquire
416-013 655nm >90% 200-800nm OD6 / 20nm Inquire Inquire
416-015 635nm >90% 200-800nm OD6 Cy5 Ex 20nm Inquire Inquire
416-017 365nm >90% 200-800nm OD6 / 10nm Inquire Inquire
416-018 615nm >90% 200-800nm OD6 / 20nm Inquire Inquire

Fluorescence Filters are critical components used in biomedical and life science instruments. Their prior function is to separate and select the characteristic spectral bands of excitation and emitted fluorescence of the tested substance in biomedical fluorescence analysis instruments. These filters find widespread applications in fields such as Point-of-Care Testing (POCT), Polymerase Chain Reaction (PCR)fluorescence enzyme labeling instruments, and fluorescence microscopes. Fluorescence filters consist of two to three parts: excitation filtersemission filters (also known as barrier filers), and, depending on the optical path requirements, dichroic filters might be employed. 

During the operation process, the excitation filter is placed within the illumination path of a fluorescence microscope and transmits a narrow band of light around the peak fluorophore excitation wavelength (i.e. the wavelengths that excite a specific dye) toward the specimen. The emission filter (also known as the barrier filter) selects the fluorescence emitted from the sample while removing other unwanted traces of excited light. Barrier filters block (suppress) shorter wavelengths and have high transmission for longer wavelengths. Emission filters can be either bandpass filters or long-pass filters, depending on the specific requirements of the application. A dichroic filter (or beamsplitter) is placed at a 45° angle in the microscope's optical path. It reflects one color of light (excitation light) and transmits another color of light (emission light).  In a microscope, the dichroic mirror directs the proper wavelengths to the specimen and to the image plane. Ideal dichroic filters and beamsplitters have sharp transitions between maximum reflection and maximum transmission, with a >95% reflection for the bandwidth of the excitation filter and a transmission of >90% for the bandwidth of the emission filter.

Shalom EO offers a large selection of fluorescence filters, the fluorescence filters are crafted with standardized dimensions compatible with filter cubes produced by elite suppliers for convenient installation. Our fluorescence filters can support miscellaneous common fluorophores such as FAM EX, Alexa Fluor, ATTO, BCECF, Cy5, Cy3, DAPI, DyLight, GFP, FITC, LysoTracker, mCherry, MitoTracker, Qdot, Quasar, Rhodamine, Texas Red, etc. Shalom EO’s fluorescence filters consist of dielectric coatings deposited on glass substrates, through the meticulous engineering of varied refractive indices and reflection characteristics of the filter, high transmission within the transmission band and exceptional optical densities greater than OD6 are obtained, providing effective spectral selection strength. These filters are designed to pass or reject wavelengths of light with great selection performance. High-precision polishing and processing are implemented in our state-of-the-art production line to ensure optimized spectral separation. 

 

The following table shows the suggested fluorescence filter combinations used for some common fluorescent fluorophores, if you have requirements or need more detail please contact us:

fluorescent dyeExcitation wavelengthEmission wavelengthExcitation filterExcitation filterDichroic Filter
Peak value(nm)Peak value(nm)CWL(nm)FWHM(nm)CWL(nm)FWHM(nm)High reflective range (nm)High Transmission Range (nm)
Alexa Fluor® 3503424423505046050330-380430-700
Alexa Fluor® 4884995204804053550400-500520-700
Alexa Fluor® 5145185435004056050450-510530-650
Alexa Fluor® 5325315545303057540450-540560-650
Alexa Fluor® 5465565725452559030450-540570-700
Alexa Fluor® 5555535655452559030450-540570-700
Alexa Fluor® 5685796035702561540500-580600-700
Alexa Fluor® 5945906185802562530500-590610-700
Alexa Fluor® 6336316476055067050550-620645-750
Alexa Fluor® 6476536686403069050600-650665-730
Alexa Fluor® 6806797026504572060600-660690-800
Alexa Fluor® 7006967196504572060600-660690-800
Alexa Fluor® 7507517767107581090600-730770-900
AmCyan4584894352048040425-445460-600
APC (Allophycocyanin)6506606206070075600-650665-730
AsRed 25765925453062060530-560580-700
ATTO 3903924773952546050350-410430-550
ATTO 4254364844453050040400-460480-600
ATTO 4654535074704052550400-480500-600
ATTO 4885005254804053550400-500520-600
ATTO 5505535765452059030450-550570-700
ATTO 6336296586055067050550-620640-750
ATTO 647N6456686206070075590-650665-735
ATTO 6806807006504572060600-670690-800
BCECF (pH 5.2)4825204804054040350-500520-650
BCECF (pH 9.0)5025284804054040350-500520-650
Biosearch Blue3524473505046050330-390430-550
BODIPY FL/pH7.25055124804053550400-500520-600
Brilliant Violet™ 4214084223952543030350-405415-500
Brilliant Violet™ 4804374784352048030425-445460-600
CAL Fluor® Gold 5405225445303057540450-540555-650
CAL Fluor® Orange 5605385595303057540450-540555-650
CAL Fluor® Red 5905695915702561540500-580595-700
CAL Fluor® Red 6105906105802562530500-590605-700
CAL Fluor® Red 6356186376055067050550-620645-750
Calcein4945174804053550400-500515-600
Calcium Green™-15075294804053550400-500515-600
Cerulean4344734352048040425-445460-600
CFP4334754352048040425-445460-600
Citrine5145295002053530480-505520-650
Coumarin3604483505046050330-390430-550
Cy2™4925074804053550400-500515-600
Cy3.5™5785915702561540500-580595-700
Cy3™5545665452560570450-550570-700
Cy5.5™6756956504572060600-660690-800
Cy5™6496666206070075590-650665-735
Cy7™7577817107581090600-730770-900
DAPI3594613505046050330-390430-550
Di-8-ANEPPS non-ratiometric4666304803062060400-495515-700
DiA4575864803062060400-495515-700
DiD6486706206070075590-650665-735
DiI5515695452560570450-550570-700
DiO4905064704052550400-480500-600
DiR7507827107581090600-730770-900
Draq56476836206070075590-650665-735
DsRed5595835452560570450-550570-700
DyLight 3503514343505046050330-390430-550
DyLight 4053984204052046050350-410430-550
DyLight 5495535695452560570450-550570-700
DyLight 5945936185802562530500-590605-700
DyLight 6336206496055067050550-620645-750
DyLight 6496516736206070075590-650665-735
DyLight 6806767056504572060600-660690-800
DyLight 7507517787107581090600-730770-900
DyLight 8007707957404080040650-750780-900
EBFP23854483952546050350-410430-550
ECFP4344774352048040425-445460-600
EGFP4885074704052550400-480500-600
Emerald GFP4895104704052550400-480500-600
Eosin5255465004056050450-510530-650
Ethidium bromide5186035453062060530-560580-700
Ethidium homidimer-1/DNA5276175453062060530-560580-700
EYFP/pH 75145275002053530480-505520-650
FAM4925184804053550400-500515-600
FITC4955194804053550400-500515-600
FlAsH-CCPFCC5115305002053530480-505520-650
Fluo-35065275002053530480-505520-650
Fluo-44945164804053550400-500515-600
FM™ 1-434735794703058060400-495515-700
FM™ 4-6450873051040730100450-590605-800
Fura-2/Ca2+ - free3665153655051080330-390430-700
Fura-2/Ca2+ - saturated3365053353051080320-390430-700
FusionRed5776045604063075500-570595-750
GFP4895114704052550400-480500-600
HEX,SE5335595303057540450-540555-700
Hoechst 332583524553505046050330-390430-700
Indo-1/Ca2+ -free3464803505046050330-390430-700
Indo-1/Ca2+ -saturated3304003504042040310-380395-550
JOE5205485004056050450-510530-650
Killer Red5856115604063075500-570595-750
Li-Cor IRDye® 680LT6746936504572060600-660690-800
Li-Cor IRDye® 800CW7787947404080040650-750780-900
Lucifer yellow4285444204053540390-445460-600
LysoTracker Blue3734223952546050350-410430-550
LysoTracker Green5015094704052550400-480500-600
LysoTracker Red5735925604063075500-570595-750
LysoTracker Yellow HCK-1234885655004056050450-510530-700
mCherry5876105604063075500-570595-750
mCitrine5145295002053530480-505520-650
MitoTracker™ Deep Red6416626206070075590-650665-735
MitoTracker™ Green4905124704052550400-480500-600
MitoTracker™ Orange5515755502059030450-550570-700
MitoTracker™ Red5785985604063075500-570595-750
mKate25886335604063075500-570595-750
mKO5495595303057540450-540555-700
mOrange25515655303057540450-540555-700
mPlum5896495604065040450-590605-800
mRFP15906125604063075500-570595-750
mTFP14624924453050040400-460480-600
mWasabi4935094704052550400-480500-600
NBD-X (MeOH)4675384804053550400-500515-700
Nile blue6316606206070075590-650665-735
NirFP6046696004067040580-620650-800
Oregon Green™ 4884985264804053550400-500515-700
Oregon Green™ 5145135325002053530480-505520-650
Pacific Blue™4044554052046050350-410430-550
Propidium Iodide (PI)3056175453062060530-560580-700
Pulsar™ 6504606504404063075400-480500-600
Qdot® 525 Nanocrystals3005254255052530370-470490-700
Qdot® 545 Nanocrystals3005434255054530370-470490-700
Qdot® 565 Nanocrystals3005644255056530370-470490-700
Qdot® 585 Nanocrystals3005884255058530370-470490-700
Qdot® 605 Nanocrystals3006024255060530370-470490-700
Qdot® 625 Nanocrystals3006214255062530370-470490-700
Qdot® 655 Nanocrystals3006544255065530370-470490-700
Qdot® 705 Nanocrystals3007024255070530370-470490-700
Qdot® 800 Nanocrystals3007924255080040370-470490-700
Quasar® 5705485665303057540450-540555-700
Quasar® 6706476706206070075590-650665-735
Quasar® 7056907506504572060600-660690-800
ReAsH-CCPGCC5926065604063075500-570595-750
Resorufin5715845452059030450-550570-700
Rhod-25535775452560570450-550570-700
Rhodamine 1235075295002053530480-505520-650
Rhodamine 6G5255475004056050450-510530-700
Rhodamine Red-X5735915604063075500-570595-750
ROX5786045604063075500-570595-750
R-phycoerythrin5655785451058540500-550570-800
SBFI/Na+ -free34056035040560100330-390430-700
SBFI/Na+ -saturated33752735040560100330-390430-700
Sulforhodamine 101-EtOH5785935604063075500-570595-750
SYBR® Green I4985224804053550400-500515-700
SYTO 606506816206070075590-650665-735
SYTO 94835004704052550400-480500-600
TagBFP4024574052046050350-410430-550
TagRFP5565845452560570450-550570-700
TAMRA5555805452560570450-550570-700
tdTomato5545815452560570450-550570-700
TET5215425004056050450-510530-700
Tetracycline4055504054055060350-460480-650
Tetramethylrhodamine dextran5545825452560570450-550570-700
Texas Red®5926145604063075500-570595-750
TO-PRO™-36426576206070075590-650665-735
TRITC5525785452560570450-550570-700
TurboFP6505926505604063075500-570595-750
wtGFP4745094505052550370-470490-700
X-Rhod-1 Indicator5806015604063075500-570595-750
ZsGreen14935054704052550400-480500-600
ZsYellow15295395004056050450-510530-700

Specifications:

Excitation Filter:

Substrate MaterialsCustomTransmission Central Wavelength RangeCan be tailored upon request
Diameters6-25.4mm 
MountMounted
CoatingHard coating


Emission Filter:

Substrate MaterialsCustomTransmission Central Wavelength RangeCan be tailored upon request
Diameters6-25.4mm 
MountMounted
CoatingHard coating


Dichroic Filter:

Substrate MaterialsCustomReflection BandCan be tailored upon request
DiametersCan be tailored upon request 
Transmission BandCan be tailored upon request
CoatingAR coating


Fluorescence Filter CW615nm, BW20nm, OD6

Fluorescence Filter CW615nm, BW20nm, OD6

Fluorescence Filter CW365nm, BW10nm, OD6

Fluorescence Filter CW365nm, BW10nm, OD6

FAM Em Fluorescence Filter, CW 525nm, BW 20nm, OD6

FAM Em Fluorescence Filter, CW 525nm, BW 20nm, OD6